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blue fluorescent

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By Targets:	Fluorescent Dye (24) Antibiotic (1) Bacterial (1) Biochemical Assay Reagents (1) Cytochrome P450 (1) DNA Stain (1) Fungal (1) Wnt (1)
By Research Areas:	Cancer (1) Infection (2) Metabolic Disease (2) Neurological Disease (2)

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Fluorescent Dye

MCE Kits

Natural
Products

Targets Recommended: Fluorescent Dye

Cat. No.	Product Name	Target	Research Areas	Chemical Structure

HY-D0068

DEAC, SE 7-Diethylaminocoumarin-3-carboxylic acid, SE	Fluorescent Dye	Others
DEAC, SE (7-Diethylaminocoumarin-3-carboxylic acid, SE) is an excellent blue fluorescent building block for labeling amine-containing biomolecules.

HY-W011618

N-(9-Acridinyl)maleimide	Fluorescent Dye	Others
N-(9-Acridinyl)maleimide is a maleimide type fluorescent thiol reagent. N-(9-Acridinyl)maleimide shows no substantial fluorescence but its coupling products with thiol compounds exhibit strong blue fluorescence. N-(9-Acridinyl)maleimide is used for fluorometrical analysis of cysteine and glutathione .

HY-145385

Coelenteramide	Fluorescent Dye	Others
Coelenteramide is a oxidative product of Coelenterazine (HY-18743). Coelenteramide can form a complex with apoAequorin/Ca ²⁺, which is known as blue fluorescent protein (BFP) and shows continuous weak luminescence with Coelenterazine like a luciferase. Coelenteramide can be used as an imaging agent .

HY-D0115

7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester	DNA Stain	Others
7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is the amine-reactive succinimidyl ester of 7-Hydroxycoumarin-3-carboxylic acid. 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is a blue fluorescent dye for labeling proteins and nucleic acids .

HY-D1775

LysoTracker Blue DND-22

Fluorescent Dye Others

Lysotracker blue DND-22 is a blue-fluorescent probe for staining acidic compartments in live cells.

LysoTracker Blue DND-22	Fluorescent Dye	Others
Lysotracker blue DND-22 is a blue-fluorescent probe for staining acidic compartments in live cells.

HY-D1445

LysoSensor PDMPO	Fluorescent Dye	Metabolic Disease
PDMPO, a lysosome pH indicator, is an excellent fluorescent acidotropic reagent for fluorescence imaging. PDMPO is a potent tool with which to study acidic organelles of live cells. PDMPO exhibits pH-dependent dual-excitation and dual-emission spectral peaks. PDMPO produces a blue fluorescence in weakly acidic organelles and shifts to yellow in more acidic lysosomes (Abs=329 nm; Em=440 nm) .

HY-101887

Calcein Blue 1 Publications Verification	Fluorescent Dye	Others
Calcein Blue, a membrane-impermeant fluorescent dye, is a coumarin derivative that contains an iminodiacetic acid structure. Calcein Blue is also a metallofluorochromic indicator .

HY-D1462

CellTracker Blue CMAC	Fluorescent Dye	Others
CellTracker Blue CMAC is a fluorescent dye , whose chloromethyl group can form a covalent bond with cell proteins. CellTracker Blue CMAC providing a stable attachment permitting long-term cell tracking .

HY-W110898

Nile blue chloride	Fluorescent Dye	Others
Nile blue chloride is a highly fluorescent and photostable organic dye. Nile blue chloride and fluorescein isothiocyanate (FITC) can be used to construct a ratiometric pH sensitive probe for tracking the pH of the extracellular fluid between cancer cells in realtime. Nile Blue chloride has the potential for the research of nonlinear optics .

HY-D0017

Dansyl chloride DNSCl	Fluorescent Dye	Others
Dansyl chloride is a reagent that produces stable blue or blue-green fluorescent sulfonamide adducts in the reaction of aliphatic and aromatic amines with primary amino groups, and is widely used for modified amino acids, protein sequencing and amino acid analysis .

HY-D1161

True Blue NCI 240899	Fluorescent Dye	Neurological Disease
True Blue (NCI 240899) is a fluorescent dye, as neuronal retrograde tracer (excitation wavelength 395-425 nm, barrier filter 450 nm). True Blue can label neuron and has no effects on neuronal survival .

HY-D0069

CPM

Fluorescent Dye Others

CPM is a maleimide derivative, acting as a blue fluorescent thiol-reactive dye. The excitation/emission maxima is 384/470 nm .
HY-117070

TO-PRO-3 iodide

Fluorescent Dye Others

TO-PRO-3 iodide is a highly efficient blue fluorescent dye that can stain cytoplasm as a cell tracer.

CPM	Fluorescent Dye	Others
CPM is a maleimide derivative, acting as a blue fluorescent thiol-reactive dye. The excitation/emission maxima is 384/470 nm .

TO-PRO-3 iodide	Fluorescent Dye	Others
TO-PRO-3 iodide is a highly efficient blue fluorescent dye that can stain cytoplasm as a cell tracer.

HY-D1256

Msr-blue	Fluorescent Dye	Neurological Disease Metabolic Disease
Msr-blue is a first turn-on fluorescent probe for methionine sulfoxide reductase with a more than 100-fold fluorescence increment. Msr-blue is used for monitoring the enzyme activity in live cells (λex=340 nm, λem=440 nm) .

HY-137845

4-Methylumbelliferyl β-D-galactopyranoside	Fluorescent Dye	Others
4-Methylumbelliferyl β-D-galactopyranoside is a fluorescent substrate for β-galactosidase which, when cleaved, produces a water-soluble blue fluorescent coumarin fluorophore that can be detected using a fluoroenzymeter or fluorometer .

HY-124171

Zinquin ethyl ester	Fluorescent Dye	Others
Zinquin ethyl ester is a fluorescent derivative of Zinquin and is a fluorescent probe of cytosolic zinc. Zinquin ethyl ester is able to penetrate cell membranes and is lipophilic and zinc-sensitive. Zinquin ethyl ester can combine with Zn ²⁺ in the presence of Ca ²⁺ and Mg ²⁺ to produce blue fluorescence .

HY-D0055

3-Cyano-7-ethoxycoumarin	Cytochrome P450	Others
3-Cyano-7-ethoxycoumarin is a fluorogenic cytochrome P-450 substrate that generates blue fluorescent product upon enzyme cleavage Target: Cytochrome P450 3-Cyano-7-ethoxycoumarin is a fluorescent probe useful in microsomal dealkylase studies.

HY-118540

Resazurin Diazoresorcinol	Fluorescent Dye	Others
Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal) .

HY-D1254

Trx-red NBL-SS perchlorate	Fluorescent Dye	Others
Trx-red (NBL-SS perchlorate) is a red-emitting fluorescent probe derivatized from the nile blue fluorophore. Trx-red is used for selectively imaging thioredoxin (Trx) in live cells and in vivo (λex=615 nm, λem=661 nm) .

HY-131045

HADA hydrochloride 1 Publications Verification HCC-Amino-D-alanine hydrochloride	Bacterial	Infection
HADA hydrochloride (HCC-Amino-D-alanine hydrochloride) is a blue (λ_em~450 nm) fluorescent D-amino acid (FDAA). FDAAs are efficiently incorporated into the peptidoglycans (PGs) of diverse bacterial species at the sites of PG biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation .

HY-D0025

7-Amino-4-methylcoumarin-3-acetic acid	Fluorescent Dye	Others
7-Amino-4-methylcoumarin-3-acetic acid is a fluorescent protein labelling agent. 7-Amino-4-methylcoumarin-3-acetic acid emits in the blue region (440-460 nm) on activation with UV light (350 nm) .

HY-118135

4-Methylumbelliferyl-α-D-galactopyranoside 4MU-α-Gal	Fluorescent Dye	Others
4-Methylumbelliferyl-α-D-galactopyranoside (4MU-α-Gal), a substrate for α-galactosidase A (GLA), is a blue pro-fluorogenic substrate. 4-Methylumbelliferyl-α-D-galactopyranoside forms two products, galactose and fluorescent 4MU, upon cleavage by GLA .

HY-D0079

Dihydroethidium Maximum Cited Publications 49 Publications Verification Hydroethidine; PD-MY 003	Fluorescent Dye	Others
Dihydroethidium, also known as DHE, is a peroxide indicator. Dihydroethidium penetrates cell membranes to form a fluorescent protein complex with blue fluoresces. After entering the cells, Dihydroethidium is mainly localized in the cell membrane, cytoplasm and nucleus, and the staining effect is the strongest in the nucleus. Dihydroethidium produces inherent blue fluorescence with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm; after dehydrogenation, Dihydroethidium combines with RNA or DNA to produce red fluorescence with a maximum excitation wavelength of 300 nm and a maximum emission wavelength of 610 nm. 535 nm can also be used as the excitation wavelength for actual observation .

HY-D1738

DAPI dilactate 4',6-Diamidino-2-phenylindole dilactate	Fluorescent Dye
DAPI (dilactate) is a blue fluorescent dye that preferentially binds dsDNA and binds to minor groove AT clusters. DAPI (dilactate) is combined with dsDNA, and the fluorescence was enhanced about 20-fold. DAPI (dilactate) can be used to identify the cell cycle and specifically stains the nucleus but not the cytoplasm. DAPI (dilactate) form is more soluble in water than DAPI (dihydrochloride) form.

HY-N7171

4,4'-Di-O-methylellagic acid 4,4'-DiOMEA; Nasutin C	Wnt	Cancer
4,4'-Di-O-methylellagic acid (4,4'-DiOMEA; Nasutin C) can be isolated from the Australian termites. 4,4'-Di-O-methylellagic acid is blue-fluorescent under ultra-violet light . 4,4'-Di-O-methylellagic acid inhibits colon cancer cell proliferation via the wnt signal pathway .

HY-D0915

Brilliant Blue FCF 2 Publications Verification Acid blue 9; FD&C blue No. 1; E133	Fluorescent Dye	Others
Brilliant Blue FCF is an aromatic hydrocarbon, a synthetic dye produced from petroleum and used as a colorant for food and other substances. The solution has a maximum absorption at 628 nm.

HY-D0718

Nile Red 35+ Cited Publications Nile blue A oxazone; Phenoxazone 9	Fluorescent Dye	Others
Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm .

HY-D0027

7-Amino-4-methylcoumarin Coumarin 120; AMC	Fluorescent Dye Antibiotic Fungal	Infection
7-Amino-4-methylcoumarin belongs to a group of coumarins. 7-Amino-4-methylcoumarin can be isolated from an endophytic fungus Xylaria sp., has broad antimicrobial activity. 7-Amino-4-methylcoumarin is additionally commonly used as an important laser dye emitting in the blue region, as well as a fluorescent probe enabling analyses of glycoproteins’ monosaccharides and N-linked oligosaccharides. The excitation wavelength and emission wavelength are 351 nm and 430 nm, respectively .

HY-W250148

Solvent black 5 Spirit nigrosine	Biochemical Assay Reagents	Others
Solvent black 5 (Spirit nigrosine) is a synthetic dye belonging to the family of azo dyes. Also known as oil black or naphthol black, it is dark blue-black and has excellent solubility in organic solvents. Solvent black 5 is commonly used as a colorant in various industrial applications such as printing inks, coatings and plastics. It can also be used as an indicator dye to detect the presence of metals in solution. Furthermore, due to its high absorption and emission properties in the near-infrared region, it has been used in scientific research as a fluorescent biomarker for tissues and cells. However, Solvent black 5 has been reported to have potentially toxic effects on human health and the environment and its use is therefore regulated in some countries.

Cat. No.	Product Name	Type

HY-D0068

DEAC, SE 7-Diethylaminocoumarin-3-carboxylic acid, SE	Fluorescent Dyes/Probes
DEAC, SE (7-Diethylaminocoumarin-3-carboxylic acid, SE) is an excellent blue fluorescent building block for labeling amine-containing biomolecules.

HY-W011618

N-(9-Acridinyl)maleimide	Fluorescent Dyes/Probes
N-(9-Acridinyl)maleimide is a maleimide type fluorescent thiol reagent. N-(9-Acridinyl)maleimide shows no substantial fluorescence but its coupling products with thiol compounds exhibit strong blue fluorescence. N-(9-Acridinyl)maleimide is used for fluorometrical analysis of cysteine and glutathione .

HY-145385

Coelenteramide	Fluorescent Dyes/Probes
Coelenteramide is a oxidative product of Coelenterazine (HY-18743). Coelenteramide can form a complex with apoAequorin/Ca ²⁺, which is known as blue fluorescent protein (BFP) and shows continuous weak luminescence with Coelenterazine like a luciferase. Coelenteramide can be used as an imaging agent .

HY-D0115

7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester	Fluorescent Dyes/Probes
7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is the amine-reactive succinimidyl ester of 7-Hydroxycoumarin-3-carboxylic acid. 7-Hydroxycoumarin-3-carboxylic acid N-succinimidyl ester is a blue fluorescent dye for labeling proteins and nucleic acids .

HY-D1775

LysoTracker Blue DND-22

Fluorescent Dyes/Probes

Lysotracker blue DND-22 is a blue-fluorescent probe for staining acidic compartments in live cells.

LysoTracker Blue DND-22	Fluorescent Dyes/Probes
Lysotracker blue DND-22 is a blue-fluorescent probe for staining acidic compartments in live cells.

HY-D1445

LysoSensor PDMPO	Fluorescent Dyes/Probes
PDMPO, a lysosome pH indicator, is an excellent fluorescent acidotropic reagent for fluorescence imaging. PDMPO is a potent tool with which to study acidic organelles of live cells. PDMPO exhibits pH-dependent dual-excitation and dual-emission spectral peaks. PDMPO produces a blue fluorescence in weakly acidic organelles and shifts to yellow in more acidic lysosomes (Abs=329 nm; Em=440 nm) .

HY-101887

Calcein Blue 1 Publications Verification	Fluorescent Dyes/Probes
Calcein Blue, a membrane-impermeant fluorescent dye, is a coumarin derivative that contains an iminodiacetic acid structure. Calcein Blue is also a metallofluorochromic indicator .

HY-D0017

Dansyl chloride DNSCl	Protein Labeling
Dansyl chloride is a reagent that produces stable blue or blue-green fluorescent sulfonamide adducts in the reaction of aliphatic and aromatic amines with primary amino groups, and is widely used for modified amino acids, protein sequencing and amino acid analysis .

HY-D1161

True Blue NCI 240899	Fluorescent Dyes/Probes
True Blue (NCI 240899) is a fluorescent dye, as neuronal retrograde tracer (excitation wavelength 395-425 nm, barrier filter 450 nm). True Blue can label neuron and has no effects on neuronal survival .

HY-D0069

CPM

Fluorescent Dyes/Probes

CPM is a maleimide derivative, acting as a blue fluorescent thiol-reactive dye. The excitation/emission maxima is 384/470 nm .
HY-117070

TO-PRO-3 iodide

Fluorescent Dyes/Probes

TO-PRO-3 iodide is a highly efficient blue fluorescent dye that can stain cytoplasm as a cell tracer.

CPM	Fluorescent Dyes/Probes
CPM is a maleimide derivative, acting as a blue fluorescent thiol-reactive dye. The excitation/emission maxima is 384/470 nm .

TO-PRO-3 iodide	Fluorescent Dyes/Probes
TO-PRO-3 iodide is a highly efficient blue fluorescent dye that can stain cytoplasm as a cell tracer.

HY-D1256

Msr-blue	Fluorescent Dyes/Probes
Msr-blue is a first turn-on fluorescent probe for methionine sulfoxide reductase with a more than 100-fold fluorescence increment. Msr-blue is used for monitoring the enzyme activity in live cells (λex=340 nm, λem=440 nm) .

HY-137845

4-Methylumbelliferyl β-D-galactopyranoside	Fluorescent Dyes/Probes
4-Methylumbelliferyl β-D-galactopyranoside is a fluorescent substrate for β-galactosidase which, when cleaved, produces a water-soluble blue fluorescent coumarin fluorophore that can be detected using a fluoroenzymeter or fluorometer .

HY-124171

Zinquin ethyl ester	Fluorescent Dyes/Probes
Zinquin ethyl ester is a fluorescent derivative of Zinquin and is a fluorescent probe of cytosolic zinc. Zinquin ethyl ester is able to penetrate cell membranes and is lipophilic and zinc-sensitive. Zinquin ethyl ester can combine with Zn ²⁺ in the presence of Ca ²⁺ and Mg ²⁺ to produce blue fluorescence .

HY-D0055

3-Cyano-7-ethoxycoumarin	Chromogenic Substrates
3-Cyano-7-ethoxycoumarin is a fluorogenic cytochrome P-450 substrate that generates blue fluorescent product upon enzyme cleavage Target: Cytochrome P450 3-Cyano-7-ethoxycoumarin is a fluorescent probe useful in microsomal dealkylase studies.

HY-118540

Resazurin Diazoresorcinol	Fluorescent Dyes/Probes
Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal) .

Resazurin

Diazoresorcinol

Fluorescent Dyes/Probes

Resazurin (Diazoresorcinol) is a water-soluble, non-toxic, stable, membrane-permeable blue non-fluorescent dye (faintly fluorescent). Resazurin is used as a redox indicator, can be reduced to pink, highly fluorescent Resorufin (Ex=530-560 nm, Em=590 nm) in living cells. Resazurin can be used for the detection of cell viability, toxicity, proliferation, migration and invasion in cells (human, plant and animal, bacterial and fungal) .

HY-D1254

Trx-red NBL-SS perchlorate	Fluorescent Dyes/Probes
Trx-red (NBL-SS perchlorate) is a red-emitting fluorescent probe derivatized from the nile blue fluorophore. Trx-red is used for selectively imaging thioredoxin (Trx) in live cells and in vivo (λex=615 nm, λem=661 nm) .

HY-131045

HADA hydrochloride 1 Publications Verification HCC-Amino-D-alanine hydrochloride	Dyes
HADA hydrochloride (HCC-Amino-D-alanine hydrochloride) is a blue (λ_em~450 nm) fluorescent D-amino acid (FDAA). FDAAs are efficiently incorporated into the peptidoglycans (PGs) of diverse bacterial species at the sites of PG biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation .

HY-D0025

7-Amino-4-methylcoumarin-3-acetic acid	Fluorescent Dyes/Probes
7-Amino-4-methylcoumarin-3-acetic acid is a fluorescent protein labelling agent. 7-Amino-4-methylcoumarin-3-acetic acid emits in the blue region (440-460 nm) on activation with UV light (350 nm) .

HY-D0079

Dihydroethidium

Maximum Cited Publications

49 Publications Verification

Hydroethidine; PD-MY 003

Fluorescent Dyes/Probes

Dihydroethidium, also known as DHE, is a peroxide indicator. Dihydroethidium penetrates cell membranes to form a fluorescent protein complex with blue fluoresces. After entering the cells, Dihydroethidium is mainly localized in the cell membrane, cytoplasm and nucleus, and the staining effect is the strongest in the nucleus. Dihydroethidium produces inherent blue fluorescence with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm; after dehydrogenation, Dihydroethidium combines with RNA or DNA to produce red fluorescence with a maximum excitation wavelength of 300 nm and a maximum emission wavelength of 610 nm. 535 nm can also be used as the excitation wavelength for actual observation .

HY-D1738

DAPI dilactate

4',6-Diamidino-2-phenylindole dilactate

Fluorescent Dyes/Probes

DAPI (dilactate) is a blue fluorescent dye that preferentially binds dsDNA and binds to minor groove AT clusters. DAPI (dilactate) is combined with dsDNA, and the fluorescence was enhanced about 20-fold. DAPI (dilactate) can be used to identify the cell cycle and specifically stains the nucleus but not the cytoplasm. DAPI (dilactate) form is more soluble in water than DAPI (dihydrochloride) form.

HY-D0915

Brilliant Blue FCF 2 Publications Verification Acid blue 9; FD&C blue No. 1; E133	Dyes
Brilliant Blue FCF is an aromatic hydrocarbon, a synthetic dye produced from petroleum and used as a colorant for food and other substances. The solution has a maximum absorption at 628 nm.

HY-D0718

Nile Red

35+ Cited Publications

Nile blue A oxazone; Phenoxazone 9

Fluorescent Dyes/Probes

Nile red (Nile blue oxazone) is a lipophilic stain. Nile red has environment-sensitive fluorescence. Nile red is intensely fluorescent in a lipid-rich environment while it has minimal fluorescence in aqueous media. Nile red is an excellent vital stain for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytof uorometry. Nile red stains intracellular lipid droplets red. The fluorescence wavelength is 559/635 nm .

HY-D0027

7-Amino-4-methylcoumarin

Coumarin 120; AMC

Fluorescent Dyes/Probes

7-Amino-4-methylcoumarin belongs to a group of coumarins. 7-Amino-4-methylcoumarin can be isolated from an endophytic fungus Xylaria sp., has broad antimicrobial activity. 7-Amino-4-methylcoumarin is additionally commonly used as an important laser dye emitting in the blue region, as well as a fluorescent probe enabling analyses of glycoproteins’ monosaccharides and N-linked oligosaccharides. The excitation wavelength and emission wavelength are 351 nm and 430 nm, respectively .

HY-W250148

Solvent black 5

Spirit nigrosine

Dyes

Solvent black 5 (Spirit nigrosine) is a synthetic dye belonging to the family of azo dyes. Also known as oil black or naphthol black, it is dark blue-black and has excellent solubility in organic solvents. Solvent black 5 is commonly used as a colorant in various industrial applications such as printing inks, coatings and plastics. It can also be used as an indicator dye to detect the presence of metals in solution. Furthermore, due to its high absorption and emission properties in the near-infrared region, it has been used in scientific research as a fluorescent biomarker for tissues and cells. However, Solvent black 5 has been reported to have potentially toxic effects on human health and the environment and its use is therefore regulated in some countries.

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